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KMID : 0387919940050010001
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Korean Journal of Cytopathology
1994 Volume.5 No. 1 p.1 ~ p.0
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Bronchoaveolar Lavage of Pneumocystis carinii Pneumonia: Cytological and Ultrastructural Features
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권건영
윤철희/김상표/박관규/장은숙
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Abstract
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The cytological and ultrastructural findings of Pneumocystis carinii(PC) obtained from rats by bronchoalveoiar lavage (BAL) are described. All developmental forms of the PC organisms were obtained in the lavage fluid.
Papanicolaou stain revealed conglomeration of PC as a foamy cast. The cystic walls of PC were well identified on Gomori's methenamine silver stain. Trophozoites and intracystic bodies were stained by Giemsa and Diff-Quik techniques. Some PC
organisms were seen within the alveolar macrophages.
Ultrastructurally, the cysts were almost circular in shape, and were nearly devoid of surface tubular extensions. The wall of the cyst was composed of an unit membrane, an intermediate electron lucent layer and an external electron dense
layer.
The cysts frequently contained intracystic bodies, so called sporozoites. Occasionally empty or collapsed cysts with no intracystic bodies, and precysts were found. Trophozoites were variable in size and shape with abundant tubular
extensions along the single electron dense pellicle.
BAL is a useful method for concentrating the various morphologic forms of PC organisms, and is a rapid diagnostic method for PC pneumonia.
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